Management and Organisational Behaviour Essay Example | Topics and Well Written Essays - 2750 words - 1

Management and Organisational Behaviour - Essay Example It is not only the behavior that is important in the organization, rather it is the elements that make up the behavior that determines the extent to which people are working towards the organizational goals. This essay illuminates the impact of organizational culture on people’s behavior and elaborates how cultural environment affects performance. The recent emergence of organizational culture in research also encompasses the importance of transforming organizational culture in the process of evoking organizational change. The cultural environment has crucial significance in the event of organizational change because of its importance in defining the employees’ reaction. It is not only the employee’s reaction that is important in determining organizational change but also it is the overall effectiveness of organizational culture that influences the process of change in an organization. Furthermore, this essay argues that transformation of organizational culture i s more important for the successful implementation of organizational change. This paper discusses the impact of organizational culture in affecting employee behavior and elaborates the importance of cultural change in the event of the organizational change. The essay question that it answers is â€Å"In recent years attention has shifted from the effects of the organization of work on people’s behavior to how behavior is influenced by the organizational culture. What is much more common today is the widespread recognition that organizational change is not just, or even necessarily mainly about changing the structure but often requires changing the culture too† Stewart (1999). Therefore, this essay encompasses the ever-increasing importance of cultural environment in the context of organizational behavior and organizational change.  

Week 7 essays Essay Example | Topics and Well Written Essays - 1250 words

Week 7 essays - Essay Example It must be emphasized that their differences had main ideological basis. Americans of the Revolutionary and the constitutional period wrestled with a†¦question: does every increase in the powers of the government entails a loss of liberty for the governed? Thomas Jefferson believed that it did. His †¦rival Alexander Hamilton believed it did not. 1 It was due to this opposing approach towards democracy that something that the founding fathers had missed or rather disregarded at the Convention of 1787 was thrust to the forefront. It was this rivalry of differing political ideals that lead to the formation of the two party system in the United States. that such an arrangement had its pit falls, and keeping these inherent dangers and Thomas Jefferson with a number of like-minded politicians, like James Madison, who likewise were fed up with the conservative Hamiltonian stance in United States politics, in the year 1794 formed the Democratic Republican party (which is the precursor of the present Democratic Party and was formally named so under Andrew Jackson in 1826). The ideological difference between the two parties stemmed from the character of Alexander Hamilton who was a through and through Conservative Right-Wing politician. In lay man terms he stood for status quo, which by extension meant among many other things the continuation of slavery. Thomas Jefferson on the other hand was a Liberal Left-Wing politician. Jefferson believed in the ideals of liberty, equality and fraternity, and these ideas were instrumental in the French Revolution of 1789. It would not be wrong to say that Hamilton was a reactionary while Jefferson a progressive politician. This rift between the two political ideologies was the main cause behind the famous 12th Amendment which was passed in the year 1804 and laid down the foundations of the multi-party system in the United States. Further, Thomas Jefferson’s

Transcription Section of the Central Dogma

Transcription Section of the Central Dogma Fulya TÃÅ"RKER TRANSCRIPTION: ONE OF THE KEY POINTS OF THE CENTRAL DOGMA ALTERNATIVE SPLICING It is known that the central dogma is the most important principle for an organism to carry on its lifespan. In the central dogma, there are 3 major stages: DNA replication and repair, transcription and translation. DNA replication can be simply described as the duplication of DNA. Transcription is the process of conversion DNA to mRNA. Last step is the translation which means the production of polypeptides from the mRNA, it is simply called as protein synthesis. In this essay, transcription section of the central dogma will be explained with detail. Why transcription is essential for living organisms? Without transcription there is no way to express genes. In order to synthesise protein, at first the mRNA should be formed from a DNA template. Proteins are the functional units in the cells which determine the phenotype of the living organisms. Since the polypeptide chains are formed from the mRNA, there should be a mechanism to create mRNA since it is not found in the cells initially. The proteins that are used in the determination of the phenotype carries the information of the used template DNA strand. DNA sequence is converted to mRNA sequence which then calls the amino acids according to this information. The amino acids form peptide bond with each other and at the end create one, long polypeptide chain. In order to initiate the process, there should be opening of two DNA strands. Since DNA is found as the double helix in the cells, when they are tightly bound to each other, RNA polymerase (the enzyme that adds nucleotides in the 3’ end of the newly formed mRNA strands) can’t bind its template and so transcription can’t initiate. So, the cell must find a way to break the hydrogen bonds between the bases of the DNA strands. In the process of transcription, the essential element is an enzyme, RNA polymerase. What is the significance of this enzyme? AT first, it helps DNA strand to open up for a specific place. The strands are separated from each other and one of them will be selected by polymerase in order to bind and use the information. The newly formed RNA will be carrying the complementary base pairs of the strand that it binds and will have the same sequence with the other strand of the DNA. These are really important points. Of course the new strand will be RNA and eventually will carry uracil instead of thymine. Secondly, in order to elongate the RNA strand, there should be addition of ribonucleotides. During DNA replication, since we are creating new DNA strand, DNA polymerase is responsible for addition of deoxyribonucleotide. However, in transcription we are concerning about RNA strand production. That’s why our enzyme should use ribonuclrotides (adenine, guanine, cytosine, thymine). The hydrogen bonds that are formed between the RNA and DNA strands are very unstable. That’s why RNA polymerase only allows very small length of binding sequence. Also, another important question is that: how the energy for RNA polymerase to move is provided? Our ribonucleotides are carrying three phosphate molecules. That’s why, they are called adenosine triphosphate or guanine etc. When they are added to the newly formed strand, they will release two of the phosphate in their bases and provide the energy necessary for RNA polymerase. There are some significant differences between the RNA polymerase and DNA polymerase except that one is taking role in replication the other is the enzyme for transcription. As indicated before, DNA polymerase is taking place in the addition of deoxyribonucleotides in the replicated DNA, while RNA polymerase adds ribonucleotides in the newly transcribed RNA molecule. Also, DNA polymerase needs a primer in order to initiate the transcription, but for RNA polymerase it is not necessary. Lastly, in the replication, the repair mechanism is highly active since we are duplicating the DNA, the errors shouldn’t be tolerated that much, but during the transcription there might be some tolerance to mistakes since after the transcription the deformed or faulty mRNA will be recognized by a mechanism and be degraded. There are five major classes of RNAs. First one is the mRNA (messenger RNA). It is so much important since it is the template and carries the genetic information for the protein synthesis. In the cell, it can be found in really small amounts. The importance of the function and the amount of it is inversely proportional in this case. Second one is the rRNA (ribosomal RNA). This is the most abundant RNA type in the living organisms’ cells. As the name is indicating, this RNA type is responsible for the structural design of the ribosomes. Ribosome (which is the place where the translation occurs) is mostly composed of rRNAs and the ribosomal proteins. Ribosome formed in the nucleolus by the association of the rRNA and ribosomal proteins. Third one is also really important for the protein synthesis to occur, it is the most important adaptor in the living organisms’ cells. It is tRNA (transfer RNA); include anticodons which recognises the codons on the mRNA and then attach t o the corresponding protein and brings the amino acid to the ribosome structure and helps forming of the correctly ordered polypeptide chain. Fourth one is the snRNA that is responsible for the splicing of premature mRNA. The other types of RNAs are providing various benefits to the cell and take role in the cellular activities. It is known that the elongation of the new strand should be from 5’ to 3’. So this newly formed strand should use the template that is moving from 3’ to 5’. By pairing with the 3’ end, its initial point will be 5’ and it will elongate through 3’. According to the direction of the movement of RNA polymerase, the mechanism will decide which strand (bottom or top strand) will be used by looking for the 3’ end of the DNA template. Since there are different kinds of RNAs are formed, there should also be different type of RNA polymerase in eukaryotic cells. RNA polymeraseI is used in the transcription of specific rRNA molecules. RNA polymerase is used in order to produce genes that carries the information for protein synthesis (including mRNA) and also snRNA. Lastly RNA polymerase III transcribes the tRNA molecules, some rRNA and snRNA. The mechanism of the transcription is a little bit complicated. Because there are lots of additional proteins are involved in the process, without them transcription can’t occur in eucaryotes. There will be comparison between prokaryotic and eukaryotic transcription machinery in the following parts of this essay. Let’s start with the transcription of the eukaryotes since it is much more complicated when it is compared to the prokaryotic system. At first, for the initiation of transcription in the eukaryotic gene has a specific sequence which is called as promoter and composed of TATA sequence. This sequence has an essential role in the transcription Ã…Å ¸n order to initiate the process. This TATA sequence is around 25 base pairs upstream from the initiator site and RNA polymerase II recognizes this site and can binds there with the help of protein complex which is called as transcription factors .The first general factor that is used in the process is TFIID and cont ains a specific part that is called TBP (TATA binding protein). TBP will fit the TATA box and activates the addition of other general transcription factor binding. When they are added to the complex RNA polymerase (contains other transcription factor on it) will be able to bind to the start site. Another important factor in the transcription initiation is the activator proteins. Those areTFIIH has a key role in this process since its ability to give the signals for the unwinding of the DNA strands by hydrolysis of the ATP. This ATP usage by TFIIH causes some modification to occur on the RNA polymerase (mostly phosphorylation). This process changes the RNA polymerase’s shape and allows the detachment of the transcription factors from the complex so from now on the initiation of the transcription ends and elongation process is ready to start. For the transcription initiation to carry on, there are some specific sequence on the genome that are called â€Å"enhancers†. Enhancers have a specific property, they are the site for activator protein binding. Enhancers may be thousands of base pair away from the RNA polymerase binding site however it has a specific ability to bend over and find the RNA polymerase so eventually let the activator proteins to interact with the other transcriptional factors on the start site or on the RNA polymerase. In addition to that in order to create a chance for the protein complex to bind to DNA, DNA must be loosely packed. Since, in normal conditions, DNA is found in a very strictly packaged conformation, this must change. Proteins need some sequence to be bound, however if the DNA is packaged strictly in the nucleosome, the transcription factor binding is impossible. So, there are some complex processes that are responsible for the change the packed conformation of the DNA and increase the approachability of the DNA by the transcriptional factor and RNA polymerase. There are mainly two ways to accomplish this aim: chromatin remodelling complex and histone modification. Remodelling complex separates the histones from the DNA strands slightly and the DNA will have a loose conformation. Histone modification is the second way for the increasing of the deforming the packed DNA. Histone acetylation is the best known technique. Histone acetylation causes the histone proteins on the nucleosome t o release the DNA slightly and make protein binding to DNA possible. Second step of the transcription is called the elongation process. There are some elongation factors which provide the attachment of RNA polymerase to DNA throughout the transcription process. Also, they carry out the RNA polymerase and increase its tolerance to the different sequences that should be transcribed. In the elongation stage, the ribonucleotides will be added to the newly formed RNA strand and at the end there will be a termination signal which causes RNA polymerase-DNA interaction breakage and lead to the product which is called precurser mature mRNA (pre-mRNA). Eukaryotic pre-mRNA needs to be exposed to some modification and of course alternative splicing. Since our genome, most of the eukaryotic organisms’ genome is composed of coding (exon) and non-coding (intron) regions, in order to translation process to occur, the pre-mRNA must cleaved from the intron sequences. In addition to that, pre-mRNA needs to be modified and the 2 ends of the pre-mRNA must have some additional feature. This is important because the translational process can’t occur without the cap modifications. Those modifications marks the mRNA as a healthy and usable product and also help the mRNA to be transported to the cytoplasm (protein synthesis occurs in the cytoplasm) from the nucleus. Firstly, let’s indicate the cap modifications. In the pre-mRNA, there are 2 caps: 5’ cap and 3’ cap. 5’ cap should be modified by the addition of 7-methylguanosine. This procedure is activated by phosphates enzyme, guanyl transferase (GTP to GMP+PP) and methyl transferase. By addition of methylguanosine, the mRNA product is separated from the other RNA molecules and also mRNA, now, will be able to transported to the cytoplasm. 3’ cap is also exposed to additional modification: Poly Adenine tail. At the end of the mRNA product, there will be addition of adenine ribonucleotides and this sequence will prevent the degradation of the mRNA. However, the most exciting and different process that the eukaryotic pre-mRNA is faced with is the splicing. At the beginning and end of each intron, there are a specific sequences that indicates that the machinery is dealing with an intron. The 5’ end of the intron mostly contains GU and 3’ end of it contains AG. Also we have specific base in the middle of the intron, Adenine, which is also called as a branch point and gives the signal for 5’ end binding and the formation of the lariat with the help of the snRNPs. What are the snRNPs and what is their role? At first, as it is claimed in the earliest pages of this essay, there is a specific RNA type which is known as snRNA (small nuclear RNA) which are the important factors in the RNA splicing. In the splicing theory, the 5 of them play an active role: U1, U2, U4, U5, U6. Those particles recognise the exon and intron end and start points and can distinguish them so help the splicing process a lot. Each of them co ntain at least seven proteins and form snRNPs (small nuclear ribonucleoprotein) which afterwards creates a structure that is called a â€Å"spliceosome†. Now, in the following section each snRNP that is actively join the splicing process will be explained. At first, there is a BBP protein which binds to the branch point (mostly Adenine). U2 snRNP recognizes BBP binding and replaces this protein with itself and form interaction with the branch point. U2 pushes A to the outside of the sequence and allows the attack of the 5’ site to this specific base. U1 initially recognises the 5’ end of the intron. U4-U5-U6 joins the process as a triplet. U4 and U6 is dissociated from each other and U6 removes the U1 snRNP and sits onto the 5’ end. U6 and branch point interacts and come closer (the process is called first phosphoryl transfer-reaction). At the end of this process lariat formation occurs. Lastly, U5 causes exon-exon interactions and second phosphoryl-transfer reaction takes place. At the end of this process the RNA is spliced and the mature mRNA is formed. In mature mRNA, there is 5’ cap, 3’ poly a tail and no introns. From now on, this mRNA is ready to synthesise the protein (the process of translatio n). Also, there is a theory of alternative splicing which should be mentioned in the discussion about the RNA splicing subject. After the removal of the introns, some exon can leave the sequence and cause alternative sequences. In this process, no shuffling of exons is allowed, but some of them leave the track. The first exon, which carries the start codon AUG, can’t change. It must be always found in the first position of the mature mRNA. The last exon of the spliced RNA must also contain one of the stop codons (UAA,UAG, UGA). But, other exon sequences are allowed to change without shuffling. Exon orders must be preserved. The below diagram indicates the process of the alternative splicing: Lastly, it is important to mention about the differences between the eukaryotic and prokaryotic transcription. In prokaryotes, we have a special term for the DNA which is called an operon. Operon carries the information for more than one gene and transcribed by the single promoter, eventually create a single mRNA which contain more than one gene. This single mRNA will be translated and eventually produce more than one protein, this characteristic of the prokaryotic DNA is called as â€Å"polycistronic† gene. However, in eucaryotes, there is only one gene that is transcribed at once. That’s why eukaryotic organisms are called as â€Å"monocistronic†. Prokaryotic transcription is only dependent to one factor in order to hold the RNA polymerase on the DNA while the eukaryotic transcription needs so many transcription factor, additional proteins and mediators. Also, since the prokaryotic DNA is found in a loose conformation in its original form, there is no need to use additional modification to destroy the packed structure as in the eukaryotes. So prokaryotic transcription machinery doesn’t use any chromatin remodelling complex or histone modifications. Prokaryotes don’t have intron in their pre-mRNA so there is no splicing in their mRNA after it is synthesised. They are free from introns, their mRNA is composed of more than one gene. Lastly, prokaryotes don’t need additional cap modification after the transcription of the mRNA. the mRNA can be easily transcribed as soon as they are synthesised. References: Belfort, M. (1989). RNA: Catalysis, splicing, evolution. Amsterdam: Elsevier . Belotserkovskaya, R., Saunders, A., Lis, J., Reinberg, D. (n.d.). Transcription through chromatin: Understanding a complex FACT. Biochimica Et Biophysica Acta (BBA) Gene Structure and Expression, 87-99. Dahlberg, J. (1989). RNA processing. San Diego: Academic Press. Darnell, J., Lodish, H. (1990). Molecular cell biology (2nd ed.). New York: Scientific American Books :. Elliott, D., Ladomery, M. (2011). Molecular biology of RNA. Oxford: Oxford University Press. Goodrich, J. A.; Tjian, R. (1994). Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II. Cell. Latchman, D. (2004). Eukaryotic transcription factors (4th ed.). Amsterdam: Elsevier/Academic Press. Locker, J. (2001). Transcription factors. Oxford: BIOS ;. Ohyama, T. (2005). DNA conformation and transcription. Georgetown, Tex.: Landes Bioscience/Eurekah.com ;. Raven, Peter H. (2011). Biology (9th ed.). New York: McGraw-Hill. Stamm, S. (2012). Alternative pre-mRNA splicing theory and protocols. Weinheim: Wiley-Blackwell. Wagner, R. (2000). Transcription regulation in prokaryotes. Oxford: Oxford University Press.

The Meaning of Life :: Philosophy essays

In approaching the meaning of life we have to examine the nature of meaning itself.   Meaning is by definition the point, or the intended goal.   Consider the point of humans and the universe as seen from monotheistic religion.   If life and the universe is some sort of toy or form of entertainment for some prime mover, his point, his own entertainment, would then be the meaning of humans and the universe.   Consider the goals of the deities of various cultures.   Some strive for a balance between the forces of 'good' and 'evil'.   This balance seems to simply be a choice of the deity, the way he thinks it ought to be.   The concept of a prime mover as a source of the meaning of life is flawed, because in talking about an actual point to absolutely everything, we are simply considering the goals of a being more powerful than ourselves who has chosen one of many possible goals that humans can conceive of.   This is to say that, if a god like this exists, his goal for life and the universe is not necessarily valid as a meaning of life, the universe, and himself.   For instance, the Bible claims that the Christian deity created the universe and placed humans in it that they might be in awe of his power.   If this is so, why is worship the correct response?   The meaning of the universe as created by God is the entertainment of God, but what is the meaning of the larger system containing God and his creations?   We could conceive of an even 'primer' mover, but that simply takes us all the way back into the wall of infinite regression.      Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   When I first read the Bible, it struck me as neutral on the idea of worship.   The Bible flat out tells you that God created humans so that they would be in awe of him, which amounts to saying God created us to inflate his ego.   We are to God as our pets are to ourselves, sources of unconditional love.   In the book of Job, God essentially makes a gentleman's bet with Satan that Job's worship is genuine and not inspired by God's kindness.   In other words, you throw a rock at my dog and I'll swing my arm so it looks I

Is It Important to Question the Ideas and Decisions of People in Positions of Authority? Essay

Is it important to question the ideas and decisions of people in positions of authority? I believe it is. While rules are meant to be followed, there should always be a reason behind that rule. There are instances where the rule is deemed by society as â€Å"unfair† or â€Å"bias.† There are other instances where the rules can be detrimental to the people following them. Let’s take a trip back to the 60’s. Remember Rosa Parks? She was arrested for refusing to give up her seat. Remember Ruby Bridges? She, a black child, was tormented because she attened an Elementary school for white students only. Now these may seem like trivial things, but if these two, along with many other Civil Rights Activists never challenged authority, segregation in the United States may have continued up to this day. When thinking about rules that can be detrimental to society, I can’t help but remember two books that represent this idea perfectly: Battle Royale, and The Hunger Games. In both books, The government forces civilians to fight to the death in an arena, and only one contestant may live. In both books, more than one contestant broke the rules and survived the game. Had they followed the rules and â€Å"played the game†, one can only imagine the physical and mental injuries they would have endured. Disobeying authority and rebelling actually saved their lives, along with many other potentially endangered lives as well. I do believe that rules are meant to be followed, but I also believe that there should always be a reason behind them. I always question authority if I think that its decisions and intentions aren’t beneficial to me – whether those decisions or intentions are trivial or extreme.

All Summer in a Day Essay

All Summer in a Day Essay In the shorty story, All Summer in a Day, by Ray Bradbury, the author expresses a tone of enthusiasm and excitement. On the planet Venus, the sun only comes out once every seven years, and when it does, it only stays out for about two hours. Margot is a 9 year old girl who was only two years old the last time her eyes met the luminous sunlight. She has dreamt about this day ever since she made that contact, and today is finally the day that she gets to feel that warm sun beaming down on her again.Because she was so caught up in her own little creative world, dreaming about this day, Margot typically shut the rest of the world out, which included her fellow class mates. Margot’s class mates don’t like her so they pick on her, and bully her every single day, all due to Margot not wanting to play games and sing songs with them unless they have something to do with the sun coming out. As a result of their hatred for her, the students decided to loc k Margot in the closet when the teacher decides leave the room for a few minutes to prevent her from seeing the sun come out.All of the children surround the window as they know that something magnificent is about to occur. The typical rainy weather finally comes to a stop and everything becomes absolutely silent. So silent that they can hear each others presence. They can’t even hear Margot ‘s pounding on the walls of the closet doors begging to be released. As the sun at last creeps through the mist of the ugly rain clouds in the sky, the children are filled with joy and playfulness. Just then, the teacher comes back into the room and shouts â€Å"Who wants to play! All of the children race outside to play in the glorious sunlight and let the fire burn their craving bodies. Page 8 describes the terrain and environment of plant Venus at this very moment, â€Å"They stopped running and stood in the great jungle that covered Venus, that grew and never stopped growing, tumultuously, even as you watched it. It was a nest of octopi, clustering up great arms of flesh-like weed, wavering, flowering this brief spring. It was the color of rubber and ash, this jungle, from the many years without sun. It was the color of stones and white cheeses and ink, and it was the color of the moon. One girl suddenly screamed out of no where as she opened her palms to a terrifying drop of rain. This marked the childrens’ last thirty seconds of their brief, glorious spring time. When the children finally got shoved back inside in class room by the adrenaline rush of the thunderous lightening from the hideous gray sky, they remembered that Margot was still locked locked in the closet and began to feel bad as they slowly creeped open the doors to let her out. Margot had missed the chance to make her wildest dreams come true.